Ever since researchers discovered that the γ-secretase complex cleaves amyloid precursor protein (APP), they have puzzled over the precise mechanism. In particular, they cannot explain why the enzyme ...
The RIG-I-like receptor (RLR) melanoma differentiation-associated gene 5 (MDA5) plays a key role in triggering innate antiviral response during infection by RNA viruses. MDA5 activation leads to ...
ChEC-seq kinetics discriminates transcription factor binding sites by DNA sequence and shape in vivo
Chromatin endogenous cleavage (ChEC) uses fusion of a protein of interest to micrococcal nuclease (MNase) to target calcium-dependent cleavage to specific genomic loci in vivo. Here we report the ...
A quantitative kinetics study has dissected the reaction steps whereby Cas12a targets DNA. This study explains the DNA cleavage patterns measured in vivo and the observations of greater reported ...
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